Currently, the service has the following infrastructure:
FORTESSA X20 analyzer cytometer
The Becton Dickinson cytometer, model Fortessa X20, is equipped with a blue laser (488nm, 50-mW,), a red laser (633 nm, 100-mW) and a violet laser (405 nm, 50-mW).
It consists of two morphological parameter detectors, (FSC and SSC) and 14 fluorescence detectors, analyzing for each of them the area, height and width of the pulse.
It incorporates an optics based on the reflection of light conducted by fiber optics, developed and patented by BD (octagon and trigon system with PMTs coupled to fiber optics) for minimum signal loss and maximum sensitivity.
It has an automated fluid system BD FACSFlow Supply System that ensures the automated supply of system fluids and stabilizes the fluid pressure in the cytometer.
For the analysis of results, it has a software called FACSDiva.
The system includes a quality module that allows to optimize voltages and to control and monitor the operation of the system.
MACSQuant Cytometer from Miltenyi Biotec
The platform also features a MACSQuant® Flow Cytometer. It is an analyzer flow cytometer equipped with 3 wavelength excitation lasers as described below:
- 405nm. Violet Laser (40mW)
- 488nm. Blue laser (25mW)
- 635nm. Red laser (20mW)
- 2 Photomultipliers for the Violet Laser (450/50 and 525/50), where Fluorochrome signals such as VioBlue ™, Pacific Blue, Pacific Orange, CFP, BFP, DAPI, ALexaFluor405, DyeCycle Violet, BD HOrizon450 can be collected , Cascade Blue, eFluo450 and Hoeschst.
- 4 Photomultipliers for the Blue Laser (525/50, 584/40, 655-730, 750LP) that allow working with: Fluorescein (FITC), Phycoerythrin (PE), Propidium Iodide (PI), 7-Actinomycin D (7-AAD), PerCP, Alexa Fluor488,, CFSE, PE-Vio770, PE-Cy5.5, PerCPCy5.5 and PE-Cy7, as well as fluorescent proteins such as GFP, EGFP, EYFP.
- 2 detectors for the Red laser (655-730 and 750LP) that allow additional work with fluorochromes such as APC, APC-Vio770, Alexa Fluor647, eFluor660, APC-Cy7, APC-H7.
In summary, it allows simultaneous analysis of 10 parameters, 8 optical channels for the simultaneous measurement of fluorescent signals, as well as two PMTs for physical parameters proportional to size (FSC) and cell complexity (SSC).
It can also measure the very important time parameter to be able to perform kinetic analysis.
It is equipped with a robotized support for sample racks (96 plates, 24 tube rack), MACS Cooling Racks that allow keeping the samples at a temperature between 4-8 degrees for two-three hours and by a magnetic column complementary that allows cell enrichment prior to acquisition.
The MACS®Quantify Software (MQS) analysis software can be installed on any computer running Microsoft® Windows® operating system. The software allows the storage in a specific format of the cytometer (MQD) and the export of data in 3 different formats: FCS 3.0, FCS 2.0 and FCS 3.1.